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    ATCC cell lines jurkat cells atcc cat
    Cell Lines Jurkat Cells Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+jurkat+e6+1+atcc+cat/Jurkat%2C+Clone+E6-1/pm41265436-276-177-181
    Average 99 stars, based on 4461 article reviews
    cell lines jurkat cells atcc cat - by Bioz Stars, 2026-09
    99/100 stars

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    Protein Purification:

    Article Title: Immunosequencing identifies signatures of T cell responses for early detection of nasopharyngeal carcinoma.
    Article Snippet: B3) BioLegend Cat#502512; RRID: AB_315236 Biological samples Serum samples from healthy individuals This paper N/A PBMC samples from NPC patients This paper N/A Tumor samples from NPC patients This paper N/A Chemicals, peptides, and recombinant proteins Human IL-2 Peprotech Cat#AF-200-02-500UG DMEM medium Gibco Cat#C11995500BT Penicillin-streptomycin Gibco Cat#15140-122 RPMI 1640 medium Hyclone Cat#SH30809.01 HEPES Gibco Cat#15630130 Sodium pyruvate Gibco Cat#11360070 Non-essential amino acids Gibco Cat#11140050 β-mercaptoethanol Sigma-Aldrich Cat#M6250 TransIT-293 Mirus Bio Cat#MIR2700 Polybrene Sigma-Aldrich Cat#TR-1003-G Liposome YEASEN Cat#40802ES03 Human serum Gemini Cat#100-512 PBS Gibco Cat#C20012500BT 7-AAD viability staining solution BioLegend Cat#420404 Permeabilization buffer Invitrogen Cat#00-8333-56 List of peptides GenScript Table S4 Critical commercial assays ELISA kit for EBV VCA-IgA Euroimmun Cat#EI 2791-9601 A DNeasy Blood Extraction kit Qiagen Cat#69506 AllPrep DNA/RNA Mini Kit Qiagen Cat#80204 QIAGEN Multiplex PCR Plus Kit QIAGEN Cat#206152 Phusion HotStart DNA polymerase kit Phusion Cat#4464269 Agencourt® Ampure® XP beads Beckman Coulter Cat#63881 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 NEBNext Ultra mRNA Library Prep Kit NEB Cat# E7530 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32851 KAPA Library Quant Kit universal qPCR Mix Illumina KK4824 NovaSeq S4 reagent Kit Illumina Cat#20028312 PrimeSTAR Takara Cat#R045B Nucleic acid extraction kit CWBIO Cat#CMY017S (Continued on next page) ll OPEN ACCESS Article e1 Cancer Cell 43, 1–19.e1–e10, August 11, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein purification kit NucleoSpin Cat#740609.250 Illumina Infinium Asian Screening Array-24 (v1.0) Illumina Cat#20016319 Deposited data Human tumor scRNA-seq data Bei et al.31 GSE162025 Human tumor scRNA-seq data Guan et al.37 GSE150825 Bulk RNA-seq data Zhang et al.38 GSE102349 Bulk TCR-seq data This paper OMIX database: PRJCA027151 VDJdb database (20191113) https://vdjdb.cdr3.net/ https://vdjdb.cdr3.net/ Experimental models: Cell lines Jurkat E6.1 ATCC CatTIB-152 HEK-293T ATCC Cat#CRL-3216 K562 ATCC Cat#CCL-243 HepG2 ATCC Cat#HB-8065 PBMCs for retroviral transduction Sailybio Cat#SLB-HP050B COS-7 Laboratory of Zeng YX. and Xu M. N/A HK1 Laboratory of Zeng YX. and Xu M. N/A C666 Laboratory of Zeng YX. and Xu M. N/A TCR-F5-Jurkat This paper N/A TCR-ID1-Jurkat This paper N/A SCT-K562 This paper N/A TCR-expressing primary T cells This paper N/A EBV ORF-transduced APCs This paper N/A Oligonucleotides Oligonucleotide pool encoding the EBV epitopes Twist Bioscience PCR primers for SCT cDNA library: 5′-GGACCCTCCGCATCC-3′and 5′-GGACCCTCCGCATCC-3′ This paper N/A PCR primer for SCT-Forward: 5′-AATGATACGGCGA CCACCGAGATCTACACTCTT TCCCTACACGACGCTCTTCC GATCTGGCCTGCTTTGTTTG CC-3′ This paper N/A PCR primer for SCT-Reverse, 5′GTGACTGGAGTTCAGACGT GTGCTCTTCCGATCTCCTCCA CCACCGCTACCTC-3′ This paper N/A Recombinant DNA Plasmid:pCCLc-peptide-β2M-HLA-eGFP This paper N/A Plasmid:pCCLc-β2M-HLA-eGFP Dr. David Baltimore from California Institute of Technology N/A Plasmid:pLV-luciferase-puromycin Addgene plasmid # 117734 Plasmid:p3XFLAG-CMV-7-EBV gene ORFs (86 ORFs) Shair et al.36 N/A Plasmid:MSGV-LNGFR-P2ATCRα-F2A-TCRβ This paper N/A Plasmid:MSGV-CD8 Dr. David Baltimore from California Institute of Technology N/A Plasmid:pRD114 Addgene plasmid # 17576 (Continued on next page) ll OPEN ACCESSArticle Cancer Cell 43, 1–19.e1–e10, August 11, 2025 e2 ..

    RNA Sequencing:

    Article Title: Immunosequencing identifies signatures of T cell responses for early detection of nasopharyngeal carcinoma.
    Article Snippet: B3) BioLegend Cat#502512; RRID: AB_315236 Biological samples Serum samples from healthy individuals This paper N/A PBMC samples from NPC patients This paper N/A Tumor samples from NPC patients This paper N/A Chemicals, peptides, and recombinant proteins Human IL-2 Peprotech Cat#AF-200-02-500UG DMEM medium Gibco Cat#C11995500BT Penicillin-streptomycin Gibco Cat#15140-122 RPMI 1640 medium Hyclone Cat#SH30809.01 HEPES Gibco Cat#15630130 Sodium pyruvate Gibco Cat#11360070 Non-essential amino acids Gibco Cat#11140050 β-mercaptoethanol Sigma-Aldrich Cat#M6250 TransIT-293 Mirus Bio Cat#MIR2700 Polybrene Sigma-Aldrich Cat#TR-1003-G Liposome YEASEN Cat#40802ES03 Human serum Gemini Cat#100-512 PBS Gibco Cat#C20012500BT 7-AAD viability staining solution BioLegend Cat#420404 Permeabilization buffer Invitrogen Cat#00-8333-56 List of peptides GenScript Table S4 Critical commercial assays ELISA kit for EBV VCA-IgA Euroimmun Cat#EI 2791-9601 A DNeasy Blood Extraction kit Qiagen Cat#69506 AllPrep DNA/RNA Mini Kit Qiagen Cat#80204 QIAGEN Multiplex PCR Plus Kit QIAGEN Cat#206152 Phusion HotStart DNA polymerase kit Phusion Cat#4464269 Agencourt® Ampure® XP beads Beckman Coulter Cat#63881 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 NEBNext Ultra mRNA Library Prep Kit NEB Cat# E7530 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32851 KAPA Library Quant Kit universal qPCR Mix Illumina KK4824 NovaSeq S4 reagent Kit Illumina Cat#20028312 PrimeSTAR Takara Cat#R045B Nucleic acid extraction kit CWBIO Cat#CMY017S (Continued on next page) ll OPEN ACCESS Article e1 Cancer Cell 43, 1–19.e1–e10, August 11, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein purification kit NucleoSpin Cat#740609.250 Illumina Infinium Asian Screening Array-24 (v1.0) Illumina Cat#20016319 Deposited data Human tumor scRNA-seq data Bei et al.31 GSE162025 Human tumor scRNA-seq data Guan et al.37 GSE150825 Bulk RNA-seq data Zhang et al.38 GSE102349 Bulk TCR-seq data This paper OMIX database: PRJCA027151 VDJdb database (20191113) https://vdjdb.cdr3.net/ https://vdjdb.cdr3.net/ Experimental models: Cell lines Jurkat E6.1 ATCC CatTIB-152 HEK-293T ATCC Cat#CRL-3216 K562 ATCC Cat#CCL-243 HepG2 ATCC Cat#HB-8065 PBMCs for retroviral transduction Sailybio Cat#SLB-HP050B COS-7 Laboratory of Zeng YX. and Xu M. N/A HK1 Laboratory of Zeng YX. and Xu M. N/A C666 Laboratory of Zeng YX. and Xu M. N/A TCR-F5-Jurkat This paper N/A TCR-ID1-Jurkat This paper N/A SCT-K562 This paper N/A TCR-expressing primary T cells This paper N/A EBV ORF-transduced APCs This paper N/A Oligonucleotides Oligonucleotide pool encoding the EBV epitopes Twist Bioscience PCR primers for SCT cDNA library: 5′-GGACCCTCCGCATCC-3′and 5′-GGACCCTCCGCATCC-3′ This paper N/A PCR primer for SCT-Forward: 5′-AATGATACGGCGA CCACCGAGATCTACACTCTT TCCCTACACGACGCTCTTCC GATCTGGCCTGCTTTGTTTG CC-3′ This paper N/A PCR primer for SCT-Reverse, 5′GTGACTGGAGTTCAGACGT GTGCTCTTCCGATCTCCTCCA CCACCGCTACCTC-3′ This paper N/A Recombinant DNA Plasmid:pCCLc-peptide-β2M-HLA-eGFP This paper N/A Plasmid:pCCLc-β2M-HLA-eGFP Dr. David Baltimore from California Institute of Technology N/A Plasmid:pLV-luciferase-puromycin Addgene plasmid # 117734 Plasmid:p3XFLAG-CMV-7-EBV gene ORFs (86 ORFs) Shair et al.36 N/A Plasmid:MSGV-LNGFR-P2ATCRα-F2A-TCRβ This paper N/A Plasmid:MSGV-CD8 Dr. David Baltimore from California Institute of Technology N/A Plasmid:pRD114 Addgene plasmid # 17576 (Continued on next page) ll OPEN ACCESSArticle Cancer Cell 43, 1–19.e1–e10, August 11, 2025 e2 ..

    Retroviral:

    Article Title: Immunosequencing identifies signatures of T cell responses for early detection of nasopharyngeal carcinoma.
    Article Snippet: B3) BioLegend Cat#502512; RRID: AB_315236 Biological samples Serum samples from healthy individuals This paper N/A PBMC samples from NPC patients This paper N/A Tumor samples from NPC patients This paper N/A Chemicals, peptides, and recombinant proteins Human IL-2 Peprotech Cat#AF-200-02-500UG DMEM medium Gibco Cat#C11995500BT Penicillin-streptomycin Gibco Cat#15140-122 RPMI 1640 medium Hyclone Cat#SH30809.01 HEPES Gibco Cat#15630130 Sodium pyruvate Gibco Cat#11360070 Non-essential amino acids Gibco Cat#11140050 β-mercaptoethanol Sigma-Aldrich Cat#M6250 TransIT-293 Mirus Bio Cat#MIR2700 Polybrene Sigma-Aldrich Cat#TR-1003-G Liposome YEASEN Cat#40802ES03 Human serum Gemini Cat#100-512 PBS Gibco Cat#C20012500BT 7-AAD viability staining solution BioLegend Cat#420404 Permeabilization buffer Invitrogen Cat#00-8333-56 List of peptides GenScript Table S4 Critical commercial assays ELISA kit for EBV VCA-IgA Euroimmun Cat#EI 2791-9601 A DNeasy Blood Extraction kit Qiagen Cat#69506 AllPrep DNA/RNA Mini Kit Qiagen Cat#80204 QIAGEN Multiplex PCR Plus Kit QIAGEN Cat#206152 Phusion HotStart DNA polymerase kit Phusion Cat#4464269 Agencourt® Ampure® XP beads Beckman Coulter Cat#63881 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 NEBNext Ultra mRNA Library Prep Kit NEB Cat# E7530 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32851 KAPA Library Quant Kit universal qPCR Mix Illumina KK4824 NovaSeq S4 reagent Kit Illumina Cat#20028312 PrimeSTAR Takara Cat#R045B Nucleic acid extraction kit CWBIO Cat#CMY017S (Continued on next page) ll OPEN ACCESS Article e1 Cancer Cell 43, 1–19.e1–e10, August 11, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein purification kit NucleoSpin Cat#740609.250 Illumina Infinium Asian Screening Array-24 (v1.0) Illumina Cat#20016319 Deposited data Human tumor scRNA-seq data Bei et al.31 GSE162025 Human tumor scRNA-seq data Guan et al.37 GSE150825 Bulk RNA-seq data Zhang et al.38 GSE102349 Bulk TCR-seq data This paper OMIX database: PRJCA027151 VDJdb database (20191113) https://vdjdb.cdr3.net/ https://vdjdb.cdr3.net/ Experimental models: Cell lines Jurkat E6.1 ATCC CatTIB-152 HEK-293T ATCC Cat#CRL-3216 K562 ATCC Cat#CCL-243 HepG2 ATCC Cat#HB-8065 PBMCs for retroviral transduction Sailybio Cat#SLB-HP050B COS-7 Laboratory of Zeng YX. and Xu M. N/A HK1 Laboratory of Zeng YX. and Xu M. N/A C666 Laboratory of Zeng YX. and Xu M. N/A TCR-F5-Jurkat This paper N/A TCR-ID1-Jurkat This paper N/A SCT-K562 This paper N/A TCR-expressing primary T cells This paper N/A EBV ORF-transduced APCs This paper N/A Oligonucleotides Oligonucleotide pool encoding the EBV epitopes Twist Bioscience PCR primers for SCT cDNA library: 5′-GGACCCTCCGCATCC-3′and 5′-GGACCCTCCGCATCC-3′ This paper N/A PCR primer for SCT-Forward: 5′-AATGATACGGCGA CCACCGAGATCTACACTCTT TCCCTACACGACGCTCTTCC GATCTGGCCTGCTTTGTTTG CC-3′ This paper N/A PCR primer for SCT-Reverse, 5′GTGACTGGAGTTCAGACGT GTGCTCTTCCGATCTCCTCCA CCACCGCTACCTC-3′ This paper N/A Recombinant DNA Plasmid:pCCLc-peptide-β2M-HLA-eGFP This paper N/A Plasmid:pCCLc-β2M-HLA-eGFP Dr. David Baltimore from California Institute of Technology N/A Plasmid:pLV-luciferase-puromycin Addgene plasmid # 117734 Plasmid:p3XFLAG-CMV-7-EBV gene ORFs (86 ORFs) Shair et al.36 N/A Plasmid:MSGV-LNGFR-P2ATCRα-F2A-TCRβ This paper N/A Plasmid:MSGV-CD8 Dr. David Baltimore from California Institute of Technology N/A Plasmid:pRD114 Addgene plasmid # 17576 (Continued on next page) ll OPEN ACCESSArticle Cancer Cell 43, 1–19.e1–e10, August 11, 2025 e2 ..

    Transduction:

    Article Title: Immunosequencing identifies signatures of T cell responses for early detection of nasopharyngeal carcinoma.
    Article Snippet: B3) BioLegend Cat#502512; RRID: AB_315236 Biological samples Serum samples from healthy individuals This paper N/A PBMC samples from NPC patients This paper N/A Tumor samples from NPC patients This paper N/A Chemicals, peptides, and recombinant proteins Human IL-2 Peprotech Cat#AF-200-02-500UG DMEM medium Gibco Cat#C11995500BT Penicillin-streptomycin Gibco Cat#15140-122 RPMI 1640 medium Hyclone Cat#SH30809.01 HEPES Gibco Cat#15630130 Sodium pyruvate Gibco Cat#11360070 Non-essential amino acids Gibco Cat#11140050 β-mercaptoethanol Sigma-Aldrich Cat#M6250 TransIT-293 Mirus Bio Cat#MIR2700 Polybrene Sigma-Aldrich Cat#TR-1003-G Liposome YEASEN Cat#40802ES03 Human serum Gemini Cat#100-512 PBS Gibco Cat#C20012500BT 7-AAD viability staining solution BioLegend Cat#420404 Permeabilization buffer Invitrogen Cat#00-8333-56 List of peptides GenScript Table S4 Critical commercial assays ELISA kit for EBV VCA-IgA Euroimmun Cat#EI 2791-9601 A DNeasy Blood Extraction kit Qiagen Cat#69506 AllPrep DNA/RNA Mini Kit Qiagen Cat#80204 QIAGEN Multiplex PCR Plus Kit QIAGEN Cat#206152 Phusion HotStart DNA polymerase kit Phusion Cat#4464269 Agencourt® Ampure® XP beads Beckman Coulter Cat#63881 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 NEBNext Ultra mRNA Library Prep Kit NEB Cat# E7530 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32851 KAPA Library Quant Kit universal qPCR Mix Illumina KK4824 NovaSeq S4 reagent Kit Illumina Cat#20028312 PrimeSTAR Takara Cat#R045B Nucleic acid extraction kit CWBIO Cat#CMY017S (Continued on next page) ll OPEN ACCESS Article e1 Cancer Cell 43, 1–19.e1–e10, August 11, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein purification kit NucleoSpin Cat#740609.250 Illumina Infinium Asian Screening Array-24 (v1.0) Illumina Cat#20016319 Deposited data Human tumor scRNA-seq data Bei et al.31 GSE162025 Human tumor scRNA-seq data Guan et al.37 GSE150825 Bulk RNA-seq data Zhang et al.38 GSE102349 Bulk TCR-seq data This paper OMIX database: PRJCA027151 VDJdb database (20191113) https://vdjdb.cdr3.net/ https://vdjdb.cdr3.net/ Experimental models: Cell lines Jurkat E6.1 ATCC CatTIB-152 HEK-293T ATCC Cat#CRL-3216 K562 ATCC Cat#CCL-243 HepG2 ATCC Cat#HB-8065 PBMCs for retroviral transduction Sailybio Cat#SLB-HP050B COS-7 Laboratory of Zeng YX. and Xu M. N/A HK1 Laboratory of Zeng YX. and Xu M. N/A C666 Laboratory of Zeng YX. and Xu M. N/A TCR-F5-Jurkat This paper N/A TCR-ID1-Jurkat This paper N/A SCT-K562 This paper N/A TCR-expressing primary T cells This paper N/A EBV ORF-transduced APCs This paper N/A Oligonucleotides Oligonucleotide pool encoding the EBV epitopes Twist Bioscience PCR primers for SCT cDNA library: 5′-GGACCCTCCGCATCC-3′and 5′-GGACCCTCCGCATCC-3′ This paper N/A PCR primer for SCT-Forward: 5′-AATGATACGGCGA CCACCGAGATCTACACTCTT TCCCTACACGACGCTCTTCC GATCTGGCCTGCTTTGTTTG CC-3′ This paper N/A PCR primer for SCT-Reverse, 5′GTGACTGGAGTTCAGACGT GTGCTCTTCCGATCTCCTCCA CCACCGCTACCTC-3′ This paper N/A Recombinant DNA Plasmid:pCCLc-peptide-β2M-HLA-eGFP This paper N/A Plasmid:pCCLc-β2M-HLA-eGFP Dr. David Baltimore from California Institute of Technology N/A Plasmid:pLV-luciferase-puromycin Addgene plasmid # 117734 Plasmid:p3XFLAG-CMV-7-EBV gene ORFs (86 ORFs) Shair et al.36 N/A Plasmid:MSGV-LNGFR-P2ATCRα-F2A-TCRβ This paper N/A Plasmid:MSGV-CD8 Dr. David Baltimore from California Institute of Technology N/A Plasmid:pRD114 Addgene plasmid # 17576 (Continued on next page) ll OPEN ACCESSArticle Cancer Cell 43, 1–19.e1–e10, August 11, 2025 e2 ..

    Polymerase Chain Reaction:

    Article Title: Immunosequencing identifies signatures of T cell responses for early detection of nasopharyngeal carcinoma.
    Article Snippet: B3) BioLegend Cat#502512; RRID: AB_315236 Biological samples Serum samples from healthy individuals This paper N/A PBMC samples from NPC patients This paper N/A Tumor samples from NPC patients This paper N/A Chemicals, peptides, and recombinant proteins Human IL-2 Peprotech Cat#AF-200-02-500UG DMEM medium Gibco Cat#C11995500BT Penicillin-streptomycin Gibco Cat#15140-122 RPMI 1640 medium Hyclone Cat#SH30809.01 HEPES Gibco Cat#15630130 Sodium pyruvate Gibco Cat#11360070 Non-essential amino acids Gibco Cat#11140050 β-mercaptoethanol Sigma-Aldrich Cat#M6250 TransIT-293 Mirus Bio Cat#MIR2700 Polybrene Sigma-Aldrich Cat#TR-1003-G Liposome YEASEN Cat#40802ES03 Human serum Gemini Cat#100-512 PBS Gibco Cat#C20012500BT 7-AAD viability staining solution BioLegend Cat#420404 Permeabilization buffer Invitrogen Cat#00-8333-56 List of peptides GenScript Table S4 Critical commercial assays ELISA kit for EBV VCA-IgA Euroimmun Cat#EI 2791-9601 A DNeasy Blood Extraction kit Qiagen Cat#69506 AllPrep DNA/RNA Mini Kit Qiagen Cat#80204 QIAGEN Multiplex PCR Plus Kit QIAGEN Cat#206152 Phusion HotStart DNA polymerase kit Phusion Cat#4464269 Agencourt® Ampure® XP beads Beckman Coulter Cat#63881 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 NEBNext Ultra mRNA Library Prep Kit NEB Cat# E7530 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32851 KAPA Library Quant Kit universal qPCR Mix Illumina KK4824 NovaSeq S4 reagent Kit Illumina Cat#20028312 PrimeSTAR Takara Cat#R045B Nucleic acid extraction kit CWBIO Cat#CMY017S (Continued on next page) ll OPEN ACCESS Article e1 Cancer Cell 43, 1–19.e1–e10, August 11, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein purification kit NucleoSpin Cat#740609.250 Illumina Infinium Asian Screening Array-24 (v1.0) Illumina Cat#20016319 Deposited data Human tumor scRNA-seq data Bei et al.31 GSE162025 Human tumor scRNA-seq data Guan et al.37 GSE150825 Bulk RNA-seq data Zhang et al.38 GSE102349 Bulk TCR-seq data This paper OMIX database: PRJCA027151 VDJdb database (20191113) https://vdjdb.cdr3.net/ https://vdjdb.cdr3.net/ Experimental models: Cell lines Jurkat E6.1 ATCC CatTIB-152 HEK-293T ATCC Cat#CRL-3216 K562 ATCC Cat#CCL-243 HepG2 ATCC Cat#HB-8065 PBMCs for retroviral transduction Sailybio Cat#SLB-HP050B COS-7 Laboratory of Zeng YX. and Xu M. N/A HK1 Laboratory of Zeng YX. and Xu M. N/A C666 Laboratory of Zeng YX. and Xu M. N/A TCR-F5-Jurkat This paper N/A TCR-ID1-Jurkat This paper N/A SCT-K562 This paper N/A TCR-expressing primary T cells This paper N/A EBV ORF-transduced APCs This paper N/A Oligonucleotides Oligonucleotide pool encoding the EBV epitopes Twist Bioscience PCR primers for SCT cDNA library: 5′-GGACCCTCCGCATCC-3′and 5′-GGACCCTCCGCATCC-3′ This paper N/A PCR primer for SCT-Forward: 5′-AATGATACGGCGA CCACCGAGATCTACACTCTT TCCCTACACGACGCTCTTCC GATCTGGCCTGCTTTGTTTG CC-3′ This paper N/A PCR primer for SCT-Reverse, 5′GTGACTGGAGTTCAGACGT GTGCTCTTCCGATCTCCTCCA CCACCGCTACCTC-3′ This paper N/A Recombinant DNA Plasmid:pCCLc-peptide-β2M-HLA-eGFP This paper N/A Plasmid:pCCLc-β2M-HLA-eGFP Dr. David Baltimore from California Institute of Technology N/A Plasmid:pLV-luciferase-puromycin Addgene plasmid # 117734 Plasmid:p3XFLAG-CMV-7-EBV gene ORFs (86 ORFs) Shair et al.36 N/A Plasmid:MSGV-LNGFR-P2ATCRα-F2A-TCRβ This paper N/A Plasmid:MSGV-CD8 Dr. David Baltimore from California Institute of Technology N/A Plasmid:pRD114 Addgene plasmid # 17576 (Continued on next page) ll OPEN ACCESSArticle Cancer Cell 43, 1–19.e1–e10, August 11, 2025 e2 ..

    cDNA Library Assay:

    Article Title: Immunosequencing identifies signatures of T cell responses for early detection of nasopharyngeal carcinoma.
    Article Snippet: B3) BioLegend Cat#502512; RRID: AB_315236 Biological samples Serum samples from healthy individuals This paper N/A PBMC samples from NPC patients This paper N/A Tumor samples from NPC patients This paper N/A Chemicals, peptides, and recombinant proteins Human IL-2 Peprotech Cat#AF-200-02-500UG DMEM medium Gibco Cat#C11995500BT Penicillin-streptomycin Gibco Cat#15140-122 RPMI 1640 medium Hyclone Cat#SH30809.01 HEPES Gibco Cat#15630130 Sodium pyruvate Gibco Cat#11360070 Non-essential amino acids Gibco Cat#11140050 β-mercaptoethanol Sigma-Aldrich Cat#M6250 TransIT-293 Mirus Bio Cat#MIR2700 Polybrene Sigma-Aldrich Cat#TR-1003-G Liposome YEASEN Cat#40802ES03 Human serum Gemini Cat#100-512 PBS Gibco Cat#C20012500BT 7-AAD viability staining solution BioLegend Cat#420404 Permeabilization buffer Invitrogen Cat#00-8333-56 List of peptides GenScript Table S4 Critical commercial assays ELISA kit for EBV VCA-IgA Euroimmun Cat#EI 2791-9601 A DNeasy Blood Extraction kit Qiagen Cat#69506 AllPrep DNA/RNA Mini Kit Qiagen Cat#80204 QIAGEN Multiplex PCR Plus Kit QIAGEN Cat#206152 Phusion HotStart DNA polymerase kit Phusion Cat#4464269 Agencourt® Ampure® XP beads Beckman Coulter Cat#63881 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 NEBNext Ultra mRNA Library Prep Kit NEB Cat# E7530 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32851 KAPA Library Quant Kit universal qPCR Mix Illumina KK4824 NovaSeq S4 reagent Kit Illumina Cat#20028312 PrimeSTAR Takara Cat#R045B Nucleic acid extraction kit CWBIO Cat#CMY017S (Continued on next page) ll OPEN ACCESS Article e1 Cancer Cell 43, 1–19.e1–e10, August 11, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein purification kit NucleoSpin Cat#740609.250 Illumina Infinium Asian Screening Array-24 (v1.0) Illumina Cat#20016319 Deposited data Human tumor scRNA-seq data Bei et al.31 GSE162025 Human tumor scRNA-seq data Guan et al.37 GSE150825 Bulk RNA-seq data Zhang et al.38 GSE102349 Bulk TCR-seq data This paper OMIX database: PRJCA027151 VDJdb database (20191113) https://vdjdb.cdr3.net/ https://vdjdb.cdr3.net/ Experimental models: Cell lines Jurkat E6.1 ATCC CatTIB-152 HEK-293T ATCC Cat#CRL-3216 K562 ATCC Cat#CCL-243 HepG2 ATCC Cat#HB-8065 PBMCs for retroviral transduction Sailybio Cat#SLB-HP050B COS-7 Laboratory of Zeng YX. and Xu M. N/A HK1 Laboratory of Zeng YX. and Xu M. N/A C666 Laboratory of Zeng YX. and Xu M. N/A TCR-F5-Jurkat This paper N/A TCR-ID1-Jurkat This paper N/A SCT-K562 This paper N/A TCR-expressing primary T cells This paper N/A EBV ORF-transduced APCs This paper N/A Oligonucleotides Oligonucleotide pool encoding the EBV epitopes Twist Bioscience PCR primers for SCT cDNA library: 5′-GGACCCTCCGCATCC-3′and 5′-GGACCCTCCGCATCC-3′ This paper N/A PCR primer for SCT-Forward: 5′-AATGATACGGCGA CCACCGAGATCTACACTCTT TCCCTACACGACGCTCTTCC GATCTGGCCTGCTTTGTTTG CC-3′ This paper N/A PCR primer for SCT-Reverse, 5′GTGACTGGAGTTCAGACGT GTGCTCTTCCGATCTCCTCCA CCACCGCTACCTC-3′ This paper N/A Recombinant DNA Plasmid:pCCLc-peptide-β2M-HLA-eGFP This paper N/A Plasmid:pCCLc-β2M-HLA-eGFP Dr. David Baltimore from California Institute of Technology N/A Plasmid:pLV-luciferase-puromycin Addgene plasmid # 117734 Plasmid:p3XFLAG-CMV-7-EBV gene ORFs (86 ORFs) Shair et al.36 N/A Plasmid:MSGV-LNGFR-P2ATCRα-F2A-TCRβ This paper N/A Plasmid:MSGV-CD8 Dr. David Baltimore from California Institute of Technology N/A Plasmid:pRD114 Addgene plasmid # 17576 (Continued on next page) ll OPEN ACCESSArticle Cancer Cell 43, 1–19.e1–e10, August 11, 2025 e2 ..

    Recombinant:

    Article Title: Immunosequencing identifies signatures of T cell responses for early detection of nasopharyngeal carcinoma.
    Article Snippet: B3) BioLegend Cat#502512; RRID: AB_315236 Biological samples Serum samples from healthy individuals This paper N/A PBMC samples from NPC patients This paper N/A Tumor samples from NPC patients This paper N/A Chemicals, peptides, and recombinant proteins Human IL-2 Peprotech Cat#AF-200-02-500UG DMEM medium Gibco Cat#C11995500BT Penicillin-streptomycin Gibco Cat#15140-122 RPMI 1640 medium Hyclone Cat#SH30809.01 HEPES Gibco Cat#15630130 Sodium pyruvate Gibco Cat#11360070 Non-essential amino acids Gibco Cat#11140050 β-mercaptoethanol Sigma-Aldrich Cat#M6250 TransIT-293 Mirus Bio Cat#MIR2700 Polybrene Sigma-Aldrich Cat#TR-1003-G Liposome YEASEN Cat#40802ES03 Human serum Gemini Cat#100-512 PBS Gibco Cat#C20012500BT 7-AAD viability staining solution BioLegend Cat#420404 Permeabilization buffer Invitrogen Cat#00-8333-56 List of peptides GenScript Table S4 Critical commercial assays ELISA kit for EBV VCA-IgA Euroimmun Cat#EI 2791-9601 A DNeasy Blood Extraction kit Qiagen Cat#69506 AllPrep DNA/RNA Mini Kit Qiagen Cat#80204 QIAGEN Multiplex PCR Plus Kit QIAGEN Cat#206152 Phusion HotStart DNA polymerase kit Phusion Cat#4464269 Agencourt® Ampure® XP beads Beckman Coulter Cat#63881 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 NEBNext Ultra mRNA Library Prep Kit NEB Cat# E7530 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32851 KAPA Library Quant Kit universal qPCR Mix Illumina KK4824 NovaSeq S4 reagent Kit Illumina Cat#20028312 PrimeSTAR Takara Cat#R045B Nucleic acid extraction kit CWBIO Cat#CMY017S (Continued on next page) ll OPEN ACCESS Article e1 Cancer Cell 43, 1–19.e1–e10, August 11, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein purification kit NucleoSpin Cat#740609.250 Illumina Infinium Asian Screening Array-24 (v1.0) Illumina Cat#20016319 Deposited data Human tumor scRNA-seq data Bei et al.31 GSE162025 Human tumor scRNA-seq data Guan et al.37 GSE150825 Bulk RNA-seq data Zhang et al.38 GSE102349 Bulk TCR-seq data This paper OMIX database: PRJCA027151 VDJdb database (20191113) https://vdjdb.cdr3.net/ https://vdjdb.cdr3.net/ Experimental models: Cell lines Jurkat E6.1 ATCC CatTIB-152 HEK-293T ATCC Cat#CRL-3216 K562 ATCC Cat#CCL-243 HepG2 ATCC Cat#HB-8065 PBMCs for retroviral transduction Sailybio Cat#SLB-HP050B COS-7 Laboratory of Zeng YX. and Xu M. N/A HK1 Laboratory of Zeng YX. and Xu M. N/A C666 Laboratory of Zeng YX. and Xu M. N/A TCR-F5-Jurkat This paper N/A TCR-ID1-Jurkat This paper N/A SCT-K562 This paper N/A TCR-expressing primary T cells This paper N/A EBV ORF-transduced APCs This paper N/A Oligonucleotides Oligonucleotide pool encoding the EBV epitopes Twist Bioscience PCR primers for SCT cDNA library: 5′-GGACCCTCCGCATCC-3′and 5′-GGACCCTCCGCATCC-3′ This paper N/A PCR primer for SCT-Forward: 5′-AATGATACGGCGA CCACCGAGATCTACACTCTT TCCCTACACGACGCTCTTCC GATCTGGCCTGCTTTGTTTG CC-3′ This paper N/A PCR primer for SCT-Reverse, 5′GTGACTGGAGTTCAGACGT GTGCTCTTCCGATCTCCTCCA CCACCGCTACCTC-3′ This paper N/A Recombinant DNA Plasmid:pCCLc-peptide-β2M-HLA-eGFP This paper N/A Plasmid:pCCLc-β2M-HLA-eGFP Dr. David Baltimore from California Institute of Technology N/A Plasmid:pLV-luciferase-puromycin Addgene plasmid # 117734 Plasmid:p3XFLAG-CMV-7-EBV gene ORFs (86 ORFs) Shair et al.36 N/A Plasmid:MSGV-LNGFR-P2ATCRα-F2A-TCRβ This paper N/A Plasmid:MSGV-CD8 Dr. David Baltimore from California Institute of Technology N/A Plasmid:pRD114 Addgene plasmid # 17576 (Continued on next page) ll OPEN ACCESSArticle Cancer Cell 43, 1–19.e1–e10, August 11, 2025 e2 ..

    Plasmid Preparation:

    Article Title: Immunosequencing identifies signatures of T cell responses for early detection of nasopharyngeal carcinoma.
    Article Snippet: B3) BioLegend Cat#502512; RRID: AB_315236 Biological samples Serum samples from healthy individuals This paper N/A PBMC samples from NPC patients This paper N/A Tumor samples from NPC patients This paper N/A Chemicals, peptides, and recombinant proteins Human IL-2 Peprotech Cat#AF-200-02-500UG DMEM medium Gibco Cat#C11995500BT Penicillin-streptomycin Gibco Cat#15140-122 RPMI 1640 medium Hyclone Cat#SH30809.01 HEPES Gibco Cat#15630130 Sodium pyruvate Gibco Cat#11360070 Non-essential amino acids Gibco Cat#11140050 β-mercaptoethanol Sigma-Aldrich Cat#M6250 TransIT-293 Mirus Bio Cat#MIR2700 Polybrene Sigma-Aldrich Cat#TR-1003-G Liposome YEASEN Cat#40802ES03 Human serum Gemini Cat#100-512 PBS Gibco Cat#C20012500BT 7-AAD viability staining solution BioLegend Cat#420404 Permeabilization buffer Invitrogen Cat#00-8333-56 List of peptides GenScript Table S4 Critical commercial assays ELISA kit for EBV VCA-IgA Euroimmun Cat#EI 2791-9601 A DNeasy Blood Extraction kit Qiagen Cat#69506 AllPrep DNA/RNA Mini Kit Qiagen Cat#80204 QIAGEN Multiplex PCR Plus Kit QIAGEN Cat#206152 Phusion HotStart DNA polymerase kit Phusion Cat#4464269 Agencourt® Ampure® XP beads Beckman Coulter Cat#63881 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32854 NEBNext Ultra mRNA Library Prep Kit NEB Cat# E7530 Qubit dsDNA HS Assay Kit Invitrogen Cat#Q32851 KAPA Library Quant Kit universal qPCR Mix Illumina KK4824 NovaSeq S4 reagent Kit Illumina Cat#20028312 PrimeSTAR Takara Cat#R045B Nucleic acid extraction kit CWBIO Cat#CMY017S (Continued on next page) ll OPEN ACCESS Article e1 Cancer Cell 43, 1–19.e1–e10, August 11, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Protein purification kit NucleoSpin Cat#740609.250 Illumina Infinium Asian Screening Array-24 (v1.0) Illumina Cat#20016319 Deposited data Human tumor scRNA-seq data Bei et al.31 GSE162025 Human tumor scRNA-seq data Guan et al.37 GSE150825 Bulk RNA-seq data Zhang et al.38 GSE102349 Bulk TCR-seq data This paper OMIX database: PRJCA027151 VDJdb database (20191113) https://vdjdb.cdr3.net/ https://vdjdb.cdr3.net/ Experimental models: Cell lines Jurkat E6.1 ATCC CatTIB-152 HEK-293T ATCC Cat#CRL-3216 K562 ATCC Cat#CCL-243 HepG2 ATCC Cat#HB-8065 PBMCs for retroviral transduction Sailybio Cat#SLB-HP050B COS-7 Laboratory of Zeng YX. and Xu M. N/A HK1 Laboratory of Zeng YX. and Xu M. N/A C666 Laboratory of Zeng YX. and Xu M. N/A TCR-F5-Jurkat This paper N/A TCR-ID1-Jurkat This paper N/A SCT-K562 This paper N/A TCR-expressing primary T cells This paper N/A EBV ORF-transduced APCs This paper N/A Oligonucleotides Oligonucleotide pool encoding the EBV epitopes Twist Bioscience PCR primers for SCT cDNA library: 5′-GGACCCTCCGCATCC-3′and 5′-GGACCCTCCGCATCC-3′ This paper N/A PCR primer for SCT-Forward: 5′-AATGATACGGCGA CCACCGAGATCTACACTCTT TCCCTACACGACGCTCTTCC GATCTGGCCTGCTTTGTTTG CC-3′ This paper N/A PCR primer for SCT-Reverse, 5′GTGACTGGAGTTCAGACGT GTGCTCTTCCGATCTCCTCCA CCACCGCTACCTC-3′ This paper N/A Recombinant DNA Plasmid:pCCLc-peptide-β2M-HLA-eGFP This paper N/A Plasmid:pCCLc-β2M-HLA-eGFP Dr. David Baltimore from California Institute of Technology N/A Plasmid:pLV-luciferase-puromycin Addgene plasmid # 117734 Plasmid:p3XFLAG-CMV-7-EBV gene ORFs (86 ORFs) Shair et al.36 N/A Plasmid:MSGV-LNGFR-P2ATCRα-F2A-TCRβ This paper N/A Plasmid:MSGV-CD8 Dr. David Baltimore from California Institute of Technology N/A Plasmid:pRD114 Addgene plasmid # 17576 (Continued on next page) ll OPEN ACCESSArticle Cancer Cell 43, 1–19.e1–e10, August 11, 2025 e2 ..



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